Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (20X) - 500ml
Phosphate Buffered Saline (PBS) is a buffer solution widely used in biological research due to its isotonic and non-toxic nature. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for applications including protein purification, Western Blotting and immunoassays, sample dilution, and as a transport medium. This buffer is formulated in a 20X concentration, and can be diluted to support its application.
Teknova 1M Tris-HCl, pH 9.5. 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Sigma Aldrich Fine Chemicals Biosciences Dulbecco’s Phosphate Buffered Saline, 1000 mL
Dulbecco’s Phosphate Buffered Saline (DPBS) is intended for use in the maintenance of mammalian cells where a chemically defined balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. DPBS is commonly used in cell enumerations as a diluent, for rinsing cells and as a buffer in many chromatographic procedures. DPBS also is used to wash and resuspend cells during the dissociation process, where the presence of calcium and magnesium can inhibit trypsin activity. For this application, use DPBS Modified, which is formulated without calcium and magnesium salts.
Alkali Scientific QuickSilver™ Buffer Powder, Tris-Glycine-SDS Buffer Powder, 50 pouches
QuickSilverª Buffer Powder Packs offer extreme convenience and will change the way you prepare buffers in your lab. They are quick, easy to use, and come in a variety of buffers for Nucleic Acid and Protein Electrophoresis, Western Blotting, ELISA, and Cell Culture. Quick and Economical (and low shipping costs!) Convenient premeasured pouches Each pouch makes 1L of 1X buffer No pH adjustment needed Nuclease and protease free Quality and consistency guaranteed Long shelf life Buffers for Electrophoresis of Nucleic Acids and Proteins: TBE, TAE, Fast Running Buffer, Tris-Glycine-SDS, Tris-MOPS-SDS, Tris-MES-SDS Buffers for Western Blotting, ELISA and Cell Culture: TBS, TBST, PBS, PBST
Alkali Scientific CellPro™ DPBS 10X, 500ml
Balanced salt solution used for multiple cell culture applications, (liquid form). Certificate of Analysis available electronically. Standard stock items from 100 mL to 200 L Volumes up to 1000 L available as made to order Not for diagnostic or therapeutic use. For making isotonic solutions for cell or tissue rinsing, cell dilution, reagent prep, transportation etc. where maintenance of cell integrity is required for further manufacturing or research use.
Abcam Opti-Klear™ Live Cell Imaging Buffer 1 x , 60ML
Hepes based imaging buffer formulated to lower background fluorescence and maintain proper pH and osmolarity for hours at 37°C. ab275938 provides an energy source and preserves fluorescent signals.
The product is subject to the following: Abcam Restricted Use Statement
MOLTOX Molecular Toxicology Inc 0.1M Tris-HCL Buffer, pH 8.0, 1000 mL/bottle, Store at Room Temperature
MOLTOX manufactures a wide variety of buffers for your laboratory needs. All MOLTOX reagents are accompanied by a GLP-compliant Formulation and Quality Control statement.
MOLTOX Molecular Toxicology Inc Saline Solution 0.9% Dilution, 9 mL/tube, 10 tubes/box, Store at Room Temperature
MOLTOX offers a wide array of solutions and buffers for a variety of laboratory research needs. All reagents are accompanied by a GLP-compliant Formulation and Quality Control statement.
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, pH 7.4) - 1L
Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics physiological osmolarity and ion concentrations, making it ideal for applications including protein purification, Western blotting and immunoassays, sample dilution, and as a transport medium. Packaged as a 10X concentration, this solution can be diluted to support its application.
Electron Microscopy Sciences Tris Maleate Buffer 0.2M, pH range 6.4-8.0 500 ML
Please specify pH when ordering.
pH: 6.4-8.0
National Diagnostics Tris-Glycine Electroblotting Buffer (10X) 4 Liter
Tris-Glycine Electroblotting Buffer (10X) is a concentrated solution in distilled/deionized water. This buffer is intended to act as the tank buffer in Western electroblotting procedures. It is also the recommended buffer for use with nitrocellulose and nylon membranes.Tris-Glycine Electroblotting Buffer (10X) is already in solution which eliminates the weighing, mixing and adjusting of the pH necessary with powdered buffers. To prepare 1 liter of working strength buffer, add 100 ml of Tris-Glycine Electroblotting Buffer to 200 ml of methanol and 700 ml of distilled/deionized water.Tris-Glycine Electroblotting Buffer (10X) contains 0.25M Tris base and 1.92M glycine, with a pH of 8.4.